Fig. 1.
Generation of Blbp promoter- SC1-IRES-EYPm mice. (A) BLBP BAC clone (RP23-81N21), indicating the location of Blbp and nearby genes, Pkib and Smapdl3a. (B) Insertion of transgene (SCI-IRES-EYFPm-pA) into Blbp BAC clone. Integration of the transgene by homologous recombination, followed by selection of the targeted BAC clone, and removal of the Neo- cassette. BLBP-SCI-IRES-EYFPm-pA transgene thus generated was used to drive astroglial specific expression of Sc1 in the developing and mature brain. (C) Six founder lines were identified by Southern blot analysis of tail DNA and independent breeding lines were established from four (named Blbp-Sc11–4). (D) A single PCR product of ~950-bp size was detected with primers complementary to Blbp-BAC and EYFP from Blbp-Sc11–4 DNA. The transgene plasmid was used as a positive control (+ lane) and DNA isolated from wildtype mice (− lane) was used as a negative control. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]