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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Chem Phys Lipids. 2012 Jun 9;165(5):545–562. doi: 10.1016/j.chemphyslip.2012.06.001

Fig. 4.

Fig. 4

Identification of MALDI-MSI species at m/z 806.5 and m/z 834.5 with the aid of ESI-LC-MS. MALDI-MSI rat brain images of the species m/z 806.5 (A) and m/z 834.5 (B) could each be either PS or STspecies, or some combination of both. The white bar is 1 mm and the scale displays relative intensities with respect to the abundance of each ion. A total lipid extract of the serial section gives an ESI-LC-MS chromatogram (C) that separates PS and ST. LC-MS from the PS fraction (D) and ST fractions (E) show that 806.5m/z is almost entirely ST (arrows) and 834.5m/z is a mixture of PS and ST (arrows). MALDI-MS (Autoflex) and ESI-LC-MS (Q-TOF) spectra were acquired in negative mode.