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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Chem Phys Lipids. 2012 Jun 9;165(5):545–562. doi: 10.1016/j.chemphyslip.2012.06.001

Fig. 6.

Fig. 6

Oversampling to improve the spatial resolution of MALDI-MSI. A: Optical image of an H&E stain of rat brain after MALDI-MSI analysis of the regions of interest within the white boxes. Mass Spectra were collected with oversampling in both 20 and 10 micron rasters across the hippocampal area. B: MALDI-MSI image of three molecular species displaying distribution across fine structures within the hippocampus. The white scale bar is 200 microns. Different color pixels represent different species, and stronger pixel intensities indicate greater abundance with respect to each ion. Green: m/z 885.5 representing PI(38:4); Blue: m/z 885.5 representing ST(d18:1/24:0); Red: m/z 1544.8 representingGM1(d18:1/18:0). C: MALDI mass spectrum approaching cellular resolution (location indicated with arrows in panel A and inset region). White marks on H&E section resulted from laser ablation of tissue at the center of each position. MSI images were acquired with an Autoflex in negative mode.