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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Chem Phys Lipids. 2012 Jun 9;165(5):545–562. doi: 10.1016/j.chemphyslip.2012.06.001

Fig. 7.

Fig. 7

MALDI-MSI of adult rat brain tissue after DHA supplementation. Heat-map MALDI-MSI of brains from naïve (A) and DHA-dosed (B) rats showed increase in the ion at m/z 834.5 which is partly composed of PS(18:0/22:6). The white bar is 2 mm and the heat map is scaled to relative intensities with respect to the abundance of the ion at m/z 834.5. Both images are presented at the same abundance scale. As such, 100% intensity represents the highest intensity observed between both images. LC-MS profiles of CL extracts from naïve (C) and DHA-dosed (inset) serial brain sections showed an increase in DHA-containing CL marked with arrows – m/z 1570: CL(18:0)(20:4)(22:6×2); m/z 1592: CL(20:4×2) (22:6×2). Overall 29% of naïve and 38% of treated brain CL contained DHA. MALDI-MSI (Ultraflex II) and ESI-LC-MS (Q-TOF) spectra were acquired in negative mode.