Table 2.
Major CL species in pnd 17 rat brain. These 70 individual molecular species were detected as [M-H]- ions by ESI-LC-MS and as [M-2H+Na]- ions at a correspondingly greater m/z value by MALDI-MS and -MSI. If a species was previously characterized by MS/MS, its acyl chain compositions are given along with the reference: [1] = (Tyurin et al., 2008); [2] = (Bayir et al., 2007). Abundance is the relative abundance among all CL species detected as determined by ESI-LC-MS.
| Species | [M-H]- m/z |
Abundance | Acyl chains | Reference |
|---|---|---|---|---|
| CL(64:4) | 1343.94 | 0.2% | (C16:1)4 | [2] |
| CL(64:3) | 1345.91 | 0.2% | ||
| CL(64:2) | 1347.92 | 0.2% | ||
| CL(66:6) | 1367.89 | 0.2% | ||
| CL(66:5) | 1369.93 | 0.3% | ||
| CL(66:4) | 1371.94 | 0.5% | (C16:1)3/(C18:1)1 | [2] |
| CL(66:3) | 1373.96 | 0.5% | (C14:0)1/(C16:1)1/(C18:1)2 | [1] |
| CL(66:2) | 1375.97 | 0.4% | ||
| CL(66:1) | 1377.97 | 0.2% | ||
| CL(68:7) | 1393.94 | 0.4% | (C16:1)3/(C20:4)1 | [2] |
| CL(68:6) | 1395.95 | 0.6% | ||
| CL(68:5) | 1397.97 | 0.9% | (C16:1)2/(C18:1)1/(C18:2)1 | [2] |
| CL(68:4) | 1399.98 | 1.5% | (C16:1)2/(C18:1)2 | [2] |
| CL(68:3) | 1401.99 | 1.2% | (C16:1)1/(C16:0)1/(C18:1)2 | [1] [2] |
| CL(68:2) | 1404.01 | 0.7% | (C16:1)1/(C16:0)1/(C18:1)1/(C18:0)1; (C16:0)2/(C18:1)2 | [1] [2] |
| CL(68:1) | 1406.02 | 0.3% | ||
| CL(70:9) | 1417.93 | 0.2% | ||
| CL(70:8) | 1419.95 | 0.7% | ||
| CL(70:7)* | 1421.97 | 1.8% | (C16:1)1/(C18:2)3 | [2] |
| CL(70:6)* | 1423.98 | 2.1% | (C16:1)1/(C18:1)1/(C18:2)2; (C16:0)1/(C16:1)1/(C18:1)1/(C20:4)1 |
[1] [2] |
| CL(70:5)* | 1426.00 | 2.6% | (C16:1)1/(C18:2)1/(C18:1)2 | [1] [2] |
| CL(70:4)* | 1428.02 | 3.1% | (C16:1)1/(C18:1)3 | [1] [2] |
| CL(70:3)* | 1430.03 | 1.7% | ||
| CL(70:2) | 1432.04 | 0.6% | (C16:0)1/(C18:0)1/(C18:1)2 | [2] |
| CL(70:1) | 1434.04 | 0.3% | ||
| CL(72:10) | 1443.95 | 0.8% | ||
| CL(72:9) | 1445.97 | 1.5% | (C16:1)1/(C18:1)1/(C18:3)1/(C20:4)1; (C16:1)1/(C16:0)1/(C18:3)1/(C22:5)1 |
[2] |
| CL(72:8)* ** | 1447.99 | 3.6% | (C18:2)4; (C16:1)1/(C18:1)1/(C18:2)1/(C20:4)1; (C16:0)1/(C18:1)1/(C18:3)1/(C20:4)1& |
[1] |
| CL(72:7)* ** | 1450.00 | 4.8% | (C18:1)1/(C18:2)3; (C16:1)1/(C18:1)1/(C18:2)1/(C20:3)1 | [1] |
| CL(72:6)* ** | 1452.02 | 4.4% | (C18:1)2/(C18:2)2; (C16:1)1/(C18:1)2/(C20:3)1 | [2] |
| CL(72:5)* ** | 1454.03 | 4.4% | (C16:0)1/(C18:1)2/(C20:3)1; (C18:1)3/(C18:2)1 | [2] |
| CL(72:4)* | 1456.05 | 3.3% | (C18:1)4; (C16:0)1/(C18:1)2/(C20:2)1 | [2] |
| CL(72:3) | 1458.05 | 1.3% | ||
| CL(72:2) | 1460.02 | 0.4% | ||
| CL(74:12) | 1467.97 | 0.4% | ||
| CL(74:11)* | 1469.97 | 1.7% | ||
| CL(74:10)* | 1471.99 | 3.1% | ||
| CL(74:9)* ** | 1474.00 | 4.5% | (C18:1)1/(C18:2)2/(C20:4)1; (C16:0)1/(C18:1)1/(C18:2)1/(C22:6)1 |
[2] |
| CL(74:8)* ** | 1476.02 | 5.1% | (C16:1)1/(C18:1)2/(C22:5)1; (C16:1)1/(C18:2)1/(C20:3)1/(C20:2)1; (C16:0)1/(C18:1)2/(C22:6)1; (C16:0)1/(C18:1)1/(C20:4)1/(C20:3)1; (C18:1)2/(C18:2)1/(C20:4)1 |
[1] [2] |
| CL(74:7)* ** | 1478.03 | 4.2% | (C18:1)3/(C20:4)1; (C16:1)1/(C18:2)1/(C20:3)1/(C20:1)1& | [2] |
| CL(74:6)* | 1480.05 | 2.3% | (C18:1)2/(C18:0)1/(C20:4)1; (C16:0)1/(C18:1)1/(C20:4)1/(C22:4)1; (C16:0)1/(C18:0)1/(C20:4)1/(C22:5)1 |
[2] |
| CL(74:5) | 1482.06 | 1.0% | ||
| CL(74:4) | 1484.03 | 0.5% | ||
| CL(76:14) | 1491.96 | 0.3% | ||
| CL(76:13) | 1493.98 | 0.7% | (C16:1)1/(C18:2)1/(C20:4)1/(C22:6)1 | [2] |
| CL(76:12)* | 1495.99 | 2.2% | (C16:1)1/(C18:2)1/(C20:3)1/(C22:6)1 | [2] |
| CL(76:11)* ** | 1498.00 | 3.3% | (C18:1)1/(C18:2)1/(C20:4)2 | [1] [2] |
| CL(76:10)* ** | 1500.02 | 3.8% | (C18:1)2/(C20:4)2; (C18:0)1/(C18:2)2/(C22:6)1 | [1] [2] |
| CL(76:9)* ** | 1502.03 | 3.1% | (C18:1)2/(C20:4)1/(C20:3)1; (C16:0)1/(C18:1)1/(C20:4)1/(C22:4)1 |
[1] [2] |
| CL(76:8)* | 1504.05 | 1.7% | (C18:1)1/(C18:2)1/(C20:3)1/(C20:2)1; (C16:0)1/(C18:2)1/(C20:2)1/(C22:4)1 |
|
| CL(76:7) | 1506.06 | 0.8% | (C16:0)1/(C18:1)1/(C20:3)1/(C22:3)1; (C16:0)1/(C18:0)1/(C20:1)1/(C22:6)1 |
|
| CL(76:6) | 1508.04 | 0.5% | ||
| CL(78:15) | 1517.97 | 0.4% | (C16:1)1/(C18:2)1/(C22:6)2 | [1] [2] |
| CL(78:14)* | 1519.99 | 0.9% | (C18:2)1/(C20:4)3 | [1] |
| CL(78:13)* | 1522.00 | 1.6% | (C18:1)1/(C18:2)1/(C20:4)1/(C22:6)1; (C18:2)1/(C20:4)3 | [2] |
| CL(78:12)* | 1524.01 | 1.9% | (C18:1)2/(C20:4)1/(C22:6)1 | [1] [2] |
| CL(78:11)* | 1526.02 | 1.5% | (C18:1)2/(C20:3)1/(C22:6)1 | [2] |
| CL(78:10) | 1528.03 | 1.0% | (C18:1)2/(C20:4)1/(C22:4)1 | [1] |
| CL(78:9) | 1530.01 | 0.7% | ||
| CL(78:8) | 1532.04 | 0.4% | (C16:1)1/(C18:1)1/(C20:3)1/(C22:3)1; (C18:1)2/(C20:0)1/(C22:6)1 |
[2] |
| CL(80:16)* | 1543.98 | 0.5% | ||
| CL(80:15)* | 1545.98 | 0.8% | (C18:1)1/(C20:4)2/(C22:6)1 | [2] |
| CL(80:14)* ** |
||||
| cl(80:13)* ** | 1550.00 | 0.9% | (c18:1)1/(c18:0)1/(c22:6)2; (c18:1)2/(c22:5)1/(c22:6)1; (c18:0)1/(c20:4)2/(c22:5)1; (c18:2)1/(c20:3)2/(c22:5)1 ; (c18:2)1/(c18:0)1/(c22:5)1/(c22:6)1 |
[1] [2] |
| cl(80:12) ** | 1551.99 | 0.7% | (c18:0)2/(c22:6)2 | [1] [2] |
| cl(80:11) | 1554.01 | 0.6% | (c18:1)2/(c22:5)1/(c22:4)1; (c18:1)1/(c18:0)1/(c22:4)1/(c22:6)1 |
[2] |
| cl(82:18) | 1567.97 | 0.3% | ||
| cl(82:17) * | 1569.96 | 0.5% | (c18:1)1/(c20:4)1/(c22:6)2 | [1] [2] |
| cl(82:16) * | 1571.96 | 0.5% | (c18:1)1/(c20:3)1/(c22:6)2 | [2] |
| cl(82:15) | 1573.96 | 0.5% |
*
= detected by MALDI-MS
**
= detected by MALDI-MSI
&
= minor species