Figure 3. Cdc25^string Dynamics Controls Entry into Mitosis.

(A) Confocal images of His2Av-RFP and UAS-Cdc25^stringGFP (stgGFP) embryo.

(B) Average expression of UAS-Cdc25^stringGFP and of stgenh-GFPNLS reporter as a function of time from anaphase 13 (the two data sets are from two separate embryos).

(C) Nuclear and cytoplasmic concentration of Cdc25^stringGFP as a function of time from anaphase 13 (average of all the cells).

(D) Correlation between nuclear and cytoplasmic concentration derived from (C).

(E) Quantification of fluorescence intensity for individual cells.

(F) Rate of cell division as a function of Cdc25^stringGFP concentration for cells in different positions along the anterior-posterior axis. Inset shows the average expression of UAS-Cdc25^stringGFP for cells in different positions along the anterior-posterior axis.

(G) Quantification of fluorescence intensity of UAS-Cdc25^stringGFP for individual cells with time rescaled to the beginning of Cdc25^stringGFP accumulation (stg turn on, t₀).

(H) Correlation between ΔT (see Figure 2 for a definition) and the average expression rate of UAS-Cdc25^stringGFP.

(I) Fraction of cells that have divided inside mitotic domain 1 as a function of time from anaphase 13 for WT embryos (red line) and embryos heterozygous for cdc25^string (blue line). Each curve is the average of data from five different embryos. Error bars are standard error of the mean. See also Figure S2 and Movie S2.