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. Author manuscript; available in PMC: 2013 May 3.
Published in final edited form as: Dev Cell. 2012 Apr 5;22(4):763–774. doi: 10.1016/j.devcel.2012.01.019

Figure 5. Cdc25string Controls Entry into Mitosis and Cdk1 Activation in a Switch-like Manner.

Figure 5

(A) Rate of cell division as a function of IK*stg. The fit gives an apparent Hill coefficient nH = 7 ± 1.

(B) Log-log plot of the rate of cell division as a function of IK*stg.

(C) Response coefficient r as a function of IK*stg.

(D) Detection of Cdc25stringGFP and Cdk1-pY15 by western blot of individual embryos.

(E) Quantification of Cdk1-pY15 amount as a function of Cdc25stringGFP (stgGFP) amount. The line is a Hill function with coefficient nH = 3.1 ± 0.9.

(F) Rate of cell division as a function of Cdk1 activity. This dependency was derived using the dependency of the rate of cell division (A) and of Cdk1 activity (D) on Cdc25string concentration (a relation between the two different measurements of Cdc25string was inferred by measuring Cdc25stringGFP and Cdk1-pY15 levels in embryos in which approximately half of the cells were in mitosis). Error bars are standard error of the mean. See also Figure S4.