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HDAC9 is expressed in NeuN-positive neurons. Double immunostaining was carried out with anti-HDAC9 (green) and NeuN (red) for mature neurons, and counter-stained with Hoechst (blue) for nuclei. Images were taken from the hippocampal (A-F) or cortical (G-I) regions. Arrows in A-C indicated newborn nerons which were not labelled by NeuN but strongly expressing Hdac9 in the subgranular zone. DG – dentate gyrus. Bars=50um.
