Mutation in the Runx1 gene reduces LG budding and perturbs HG development in embryos at E16.5. (A–C) LG; (D–F) HG development in WT (Runx+/+) (A, D) and mutant (Runx–/–) (B, C, E, F) embryos. Yellow arrows indicate LG budding, white arrow conjunctiva, and red arrow HG epithelium component in WT embryos.