Figure 5.

Knockdown of Runx1, 2, and 3 expression results in decreased CyclinD1 protein. (A) Protein was prepared from siRNA-treated LG organ cultures, resolved by SDS-PAGE, and probed with the cyclin-D1 or β-actin antibodies. (B) Graphic representation of densitometric analyses of Western blots from two independent experiments (mean ± SD of Optical Density [OD] of bands). For analysis of the Western blot images ImageJ 1.46 (National Institutes of Health, Bethesda, MD) has been used. The relative densities of the loading-control bands and the sample bands were calculated. Adjusted density for each sample lane was calculated by dividing the sample relative density by the loading-control relative density for each lane separately. Asterisks mark significant statistical difference in the level of CyclinD1 of experimental siRNA treated lacrimal glands compared to control treated glands.