Fig. 5.

GSE24.2 peptide induces a recovery of telomerase activity and c-myc promoter activation. (a) 293T cells were transfected with the pXP (empty vector) or the pXP3.2myc promoter (0.5 µg per 35 mM dish), when indicated cells were transfected 24 hours later with the GSE24.2 peptide (15 µg per 35 mm dish). Luciferase assay was performed six hours later. The experiments were repeated 3 times, with similar results (b) Experiment was carried out as in A, but the cells were lysed after peptide transfection at the indicated times. (c, d) F9A353V cells were transfected with the GSE24.2 peptide (15 µg per 35 mm dish). Total RNA was extracted 24 hours later and the levels of mTERT (c) and c-myc (d) measured by qRT-PCR. (e) DKC1774 a human XDC cell line was transfected either with a control peptide or GSE24.2 peptide (15 µg per 35 mm dish) and telomerase activity was determined four hours later using the TRAP assay kit. Different dilutions of extracts used for each TRAP assay are indicated. Asterisks indicate the samples that showed important differences with respect to the control. The experiments were repeated 3 times, with similar results. (f) F9 and F9A353V cells were transfected either with a control peptide or GSE24.2 peptide (15 µg per 35 mm dish). Telomerase activity was determined four hours later with the TRAP assay kit. Different extract dilutions were used for each TRAP assay as indicated. Asterisks indicate the samples that showed important differences with respect to the control. The experiments were repeated 3 times, with similar results.