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. Author manuscript; available in PMC: 2013 May 3.
Published in final edited form as: Dev Biol. 2010 Dec 31;352(2):181–190. doi: 10.1016/j.ydbio.2010.12.033

Fig. 4.

Fig. 4

S100A4 induces MMP-3 expression in mammary epithelial cells grown in 3D collagen cultures. (A) Effect of endogenously expressed S100A4 and (B) effect of extracellular S100A4 (12 μg/ml) on MMP-3 expression in 3D cultures treated with TGFα, as measured by qPCR. The sh1/huA4 cell line expressed significantly higher amounts of MMP3 mRNA compared to sh scr and sh1 cells, while the sh scr and sh1 cells responded to topical addition of recombinant S100A4 by a significant increase in MMP3 mRNA. C) Western blot of conditioned medium from 3D collagen cultures of control and two different shRNA transduced cell lines treated with or without 12 μg/ml recombinant S100A4 in combination with TGFα. The immunoblot was labeled with anti MMP-3 antibody. Ponceau staining was used as loading control.