Table 1.
Data collection and refinement statistics (matched base pairs)
| PolIVdG:dCMPnPP | PolIVdC:dGMPnPP | PolIVdA:dTMPnPP | PolIVdT:dAMPnPP | |
|---|---|---|---|---|
| Data Collection | ||||
| Wavelength (Å) | 1.0 | 1.0 | 1.0 | 1.0 |
| Space group | P21 | P21 | P21 | P21 |
| Cell (Å) dimensions | 86.14, 56.95, 110.81 | 86.76, 57.04, 110.7 | 85.75, 57.25, 110.3 | 86.69, 56.88, 110.93 |
| (°) | 90.00, 93.53, 90.00 | 90.00, 94.76, 90.00 | 90.00, 90.44, 90.00 | 90.00, 93.99, 90.00 |
| Resolution (Å) | 2.67 (2.81–2.67)a | 2.33 (2.46–2.33)a | 2.38 (2.38–2.51)a | 2.48 (2.61–2.48)a |
| Rsym or Rmerge | 7.4 (52.9) | 6.1 (51.4) | 5.4 (51.8) | 5.9 (52.2) |
| I/δI | 10.7 (2.6) | 10.0 (2.2) | 12.7(2.3) | 11.3(2.3) |
| Completeness (%) | 99.7 (100) | 99.4 (99.5) | 99.9 (100) | 100 (99.9) |
| Redundancy | 3.5 (3.5) | 3.7 (3.4) | 3.6 (3.6) | 3.8 (3.8) |
| Refinement | ||||
| Resolution (Å) | 45.30–2.67 | 47.66–2.33 | 47.6–2.38 | 43.28–2.48 |
| No. of reflections | 29 306 | 44 007 | 41 474 | 36 659 |
| Rwork/Rfree | 22.5/27.5 | 22.1/27.4 | 21.2/26.1 | 22.6/27.3 |
| No. of atoms | ||||
| Protein | 5376 | 5376 | 5376 | 5376 |
| DNA | 1356 | 1356 | 1356 | 1356 |
| dNMPnPP | 58 | 62 | 60 | 56 |
| Ion | 4 | 4 | 4 | 4 |
| Water | 93 | 123 | 301 | 106 |
| R.m.s deviations | ||||
| Bond lengths (Å) | 0.011 | 0.008 | 0.007 | 0.011 |
| Bond angles (°) | 1.424 | 1.108 | 1.136 | 1.727 |
aHighest resolution shell is shown in parentheses.