Figure 5.

Dependence of the populations of the stacked-conformations (A) and the local mobility expressed as a cone semi-angle (B) of the 2-Ap residues as a function of NC(11–55) concentrations. Points from dark to light grey correspond to 0, 1, 4, 7, 10 and 15 equivalents of NC(11–55), respectively. Error bars correspond to SD of at least three independent experiments. (A) NC(11–55) generally decreased the stacked conformations, albeit to different extents, being the most efficient for residues at positions 9 and 49. (B) The cone semi-angle of the conformational space explored by 2-Ap is largely decreased by NC(11–55), when the 2-Ap residue was located in the lower part of the cTAR stem (at positions 9, 49, 53 and 55).