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. 2013 Mar 20;41(9):4792–4801. doi: 10.1093/nar/gkt169

Figure 5.

Figure 5.

TERRA accumulates in metaphases of siXRN2-treated gorilla cells. Q-FISH analyses show an accumulation of extrachromosomal telomeric signals (A) and an increase in the mean intensity of total telomere signals (B) revealed by the C-rich probe in siXRN2-treated cells. This increase is eliminated by an RNase treatment of the slides, indicating that this increase is due to TERRA accumulation. Numbers above bars indicate P-values (student t-test). On the other hand, the Q-FISH analyses also revealed the presence of telomeric doublets (sister chromatids bearing two telomeric signals, yellow arrows) in control cells, as well as in siXRN2-treated cells (C). In the latter, however, doublets were significantly more abundant, suggesting that many of these doublets were the consequence of TERRA accumulation (D). This conclusion is also supported by the fact that an RNase I treatment of the slides leads to a significant reduction of the doublets (D). At least 600 chromosomes were considered per condition. Numbers above bars indicate P-values (student t-test). N.S., no significant difference.