Table 1.
Number | Name | Sequencea |
---|---|---|
1 | 4C-G | 5′-(FAM)-AGG CAC TGA TC GGG -3′ |
3′-CC GTG ACT AG CCCCGC ATT -5′ | ||
2 | 1T-G | 5′-(FAM)-AGG CAC TGA TC GGG -3′ |
3′-CC GTG ACT AG CCCTGC ATT -5′ | ||
3 | 2T-G | 5′-(FAM)-AGG CAC TGA TC GG G -3′ |
3′-CC GTG ACT AG CCTCGC ATT -5′ | ||
4 | 3T-G | 5′-(FAM)-AGG CAC TGA TC G GG -3′ |
3′-CC GTG ACT AG CTCCGC ATT -5′ | ||
5 | 4T-G | 5′-(FAM)-AGG CAC TGA TC GGG -3′ |
3′-CC GTG ACT AG TCCCGC ATT -5′ | ||
6 | 4C-A | 5′-(FAM)-AGG CAC TGA TC GGG -3′ |
3′-CC GTG ACT AG CCCCAC ATT -5′ | ||
7 | 1T-A | 5′-(FAM)-AGG CAC TGA TC GGG -3′ |
3′-CC GTG ACT AG CCCTAC ATT -5′ | ||
8 | C-C mispair | 5′-(FAM)-AGG CAC TGA TC GGGC -3′ |
3′-CC GTG ACT AG CCCCGC ATT -5′ | ||
9 | 4C-GA | 5′-(FAM)-AGG CAC TGA TC GGG -3′ |
3′-CC GTG ACT AG CCCCGA ATT -5′ | ||
10 | 4G | 5′-(FAM)-AGG CAC TGA TC GGGG -3′ |
3′-CC GTG ACT AG CCCCGC ATT -5′ |
aPrimers are labelled on the 5′ end with 6-carboxyfluorescein (FAM).
Nucleotides in bold vary between substrates.