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. 2013 Mar 26;41(9):5127–5138. doi: 10.1093/nar/gkt201

Figure 3.

Figure 3.

The binding affinity between of DMP12 and 8mer dsDNA to Neisseria HU protein was determined by an ITC assay. To obtain good ITC data, we tested the binding between Neisseria HU and DNA substrates with different lengths (35, 21, 16 and 8mer DNA). Only the Neisseria HU/8mer DNA reaction yielded a good binding curve, and these results are similar to previous ITC data obtained by using E. coli HU and different DNA lengths (21). The 8mer DNA was, therefore, used for the DMP12 comparison. The Kd of DMP12/Neisseria HU is 2.82 ± 0.43 µM and 8mer DNA/Neisseria HU is 0.72 ± 0.06 µM. Data are from three replicate experiments. No evidence of an interaction signal was seen in the negative control titration that used the acidic protein BSA (pI 4.7) in place of DMP12 protein or dsDNA.