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. 2012 Dec;9(4):234–241. doi: 10.7497/j.issn.2095-3941.2012.04.003

Figure 3.

Figure 3

A set of miRNAs can downregulate the GNAI1 protein level by binding to its 3’ UTR. A: Schematic diagram of the predicted binding sites of the GNAI1-regulating miRNAs in the 3’ UTR of GNAI1. Several miRNAs shared the same target sites; B: Relative luciferase activity analyzed following the co-transfection of a luciferase reporter plasmid containing the WT GNAI1 3’ UTR and GNAI1-regulating miRNAs. Normalized to the Renilla luciferase activity, the data are shown as mean±SEM; C: Western blot assays of the endogenous GNAI1 protein levels in SMMC-7721 cells transfected with GNAI1-regulating miRNAs or negative control. The intensity was normalized to GAPDH, and the ratios indicate the relative expression levels compared with the negative control; D: Sequences of WT and MT (shadowed and underlined) binding sites; E: Relative luciferase activity in cells co-transfected with GNAI1-regulating miRNAs, and a luciferase reporter plasmid containing the WT or MT 3’UTR. The data are presented as mean±SEM, along with the P values.