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. Author manuscript; available in PMC: 2013 May 3.
Published in final edited form as: Comb Chem High Throughput Screen. 2011 Sep;14(8):669–687. doi: 10.2174/138620711796504442

Figure 5. Dose response of 6 confirmed Cdc7-Dbf4 kinase inhibitors that also inhibit ATP depletion and 2 false positives that inhibit luminescence detection.

Figure 5

The results of ADP production (●), ADP processing (▲), and luminescence detection (■) of the following 8 compounds in the LUM method are shown: (A). Ellagic acid; (B). Irigenol; (C). Epicatechin monogallate; (D). Epigallocatechin-3-monogallate; (E). Tannic acid; (F). Theaflavin monogallates. (G). Gentian violet; (H). Pararosaniline pamoate. The results of luminescence detection before each compound dilution was added are also shown (□). The results of ADP production are the Avg ± SE of 2 data points (n=2), and the results of ADP processing and luminescence detection are the Avg ± SE of 3 data points (n=3). Dose response curve of the average was fitted by the logistic 4-parameter equation of SigmaPlot. Solid line show the curve fit of ADP production. The IC50 values of ADP production of Ellagic acid, Irigenol, Epicatechin monogallate, Epigallocatechin-3-monogallate, Tannic acid, and Theaflavin monogallates are as follows: (A). Ellagic acid, 0.77±0.06 μM; (B). Irigenol, 1.15±0.07 μM; (C). Epicatechin monogallate, 33.66±7.55 μM; (D). Epigallocatechin-3-monogallate, 38.64±24.93 μM; (E). Tannic acid, 0.77±0.24 μM; (F). Theaflavin monogallates, 4.27±0.53 μM. The IC50 value of Ellagic acid is determined with the data of the concentration of lower than 10 μM. Short dash lines show the curve fit of luminescence detection of Tannic acid and Theaflavin monogallates. The IC50 values of luminescence detection of these compounds are greater than 77 μM. The IC50 values of ADP production, ADP processing, and luminescence detection of Gentian violet and Pararosaniline pamoate are as follows: (G). Gentian violet: 12.81±2.57 μM, 9.47±0.58 μM, 10.31±1.01 μM; (H). Pararosaniline pamoate: 14.94±4.66 μM, 9.04±0.82 μM, 11.64±1.89 μM.