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. Author manuscript; available in PMC: 2013 May 3.
Published in final edited form as: Comb Chem High Throughput Screen. 2011 Sep;14(8):669–687. doi: 10.2174/138620711796504442
Step Notes
1 to 3 Dispensing on the PP-384-M Personal Pipettor using a custom 384 head.
4 Dispensing with the FlexDrop IV.
5 Plates incubated at room temperature for 10 min.
6 Dispensing with the FlexDrop IV.
7 Plates incubated at room temperature for 2 hr.
8 Dispensing with the FlexDrop IV.
9 Plates incubated at room temperature for 1 hr.
10 Plates centrifuged at 3,000 rpm; 30 s.
11 Radiometric signal quantified with empty epi mirror and empty emission mirror. [14C]-plate as a reference.