Table 1.
List of mouse primers for real time PCR
| Cell marker | Gene | Forward primer | Reverse primer |
|---|---|---|---|
| House- keeping genes |
GAPDH |
CGTCCCGTAGACAAAATGGT |
TCAATGAAGGGGTCGTTGAT |
| β-actin |
GTGGGCCGCTCTAGGCACCAA |
CTCTTTGATGTCACGCACGATTTC |
|
| Cytokine genes |
IFN-γ |
GCGTCATTGAATCACACCTG |
TGAGCTCATTGAATGCTTGG |
| IL-1α |
TCAAGATGGCCAAAGTTCCT |
TGCAAGTCTCATGAAGTGAGC |
|
| IL-1β |
TGAAGCAGCTATGGCAACTG |
GGGTCCGTCAACTTCAAAGA |
|
| IL-2 |
AACCTGAAACTCCCCAGGAT |
CGCAGAGGTCCAAGTTCATC |
|
| IL-3 |
CCGTTTAACCAGAACGTTGAA |
CCACGAATTTGGACAGGTTT |
|
| IL-4 |
GGCATTTTGAACGAGGTCAC |
AAATATGCGAAGCACCTTGG |
|
| IL-5 |
ATGGAGATTCCCATGAGCAC |
AGCCCCTGAAAGATTTCTCC |
|
| IL-6 |
AACGATGATGCACTTGCAGA |
GGTACTCCAGAAGACCAGAGGA |
|
| IL-10 |
TGAATTCCCTGGGTGAGAAG |
TGGCCTTGTAGACACCTTGG |
|
| IL-12β |
ATCGTTTTGCTGGTGTCTCC |
CATCTTCTTCAGGCGTGTCA |
|
| IL-13 |
CCTCTGACCCTTAAGGAGCTT |
ATGTTGGTCAGGGAATCCAG |
|
| MCP-3 |
TCTGTGCCTGCTGCTCATAG |
CTTTGGAGTTGGGGTTTTCA |
|
| Growth factor genes | TGFβ2 |
GGAGGTTTATAAAATCGACATGC |
GGCATATGTAGAGGTGCCATC |
| VEGFa |
TACCTCCACCATGCCAAG |
TGGTAGACATCCATGAACTTGA |
|
| VEGFb |
GGCTTAGAGCTCAACCCAGA |
TGGAAAGCAGCTTGTCACTTT |
|
| VEGFc | GGGAAGAAGTTCCACCATCA | TCGCACACGGTCTTCTGTAA |