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. 2013 May 3;8(5):e63495. doi: 10.1371/journal.pone.0063495

Figure 5. Functional changes in cells induced by knockdown of proteins involved in mitochondrial dynamics.

Figure 5

(A and B) The mitochondrial fusion by Drp1 knockdown leads to an increase of cell length and F-actin. APRE-19 cells were transfected with Drp1 siRNA, Mfn1 siRNA, or control siRNA. Forty-eight hours after transfection, cells were treated withTGF-β for 48 h. Cells were fixed and stained with phalloidin-TRITC (A). Cell length was analyzed using confocal images (B). Data are the mean ± SD of three experiments, each with 100 cells per condition. (C) The mitochondrial fusion by Drp1 knockdown promotes cell migration induced by TGF-β. Cells were transfected with the indicated siRNAs. Forty-eight hours after transfection, cells were treated with TGF-β for the indicated periods of time. The migrated cells were counted. Data shown represent the average of three independent experiments. *P<0.05; ***P<0.0005.

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