Figure 5. Western Blotting (WB) analysis for PrP^Sc in ovine mammary glands with chronic mastitis.

Lines: 1–2 =  brain from a naturally scrapie-affected (Scr⁺) or scrapie-free (Scr^-) sheep. Lines: 3–4–8 =  mammary glands from scrapie-affected sheep displaying lymphofollicular mastitis. Lines: 5–6–7 =  mammary glands from scrapie-affected sheep displaying granulomatous mastitis. Line 9 =  mammary gland from a scrapie-affected sheep displaying lymphoproliferative/non-lymphofollicular mastitis. Line 10 =  palatine tonsil from a naturally scrapie-affected sheep showing PrP^Sc in mammary gland with lymphofollicular mastitis. Among the mammary glands, positive WB signals were observed only in the case of lymphofollicular mastitis.