Table 2. Details of the primers used to determine cytokine, chemokine, and adhesion molecule gene expression levels.
Gene | Primer sequences and orientation (5′ to 3′) | MGB-FAM probe |
LTα | Fwd-ATG GCT TCT CTC TCA GCA ACA A ; Rev-AGA CCA CTT GGG AGT AGA CGA A | CTC CCT CCT GGT CCC C |
LTβ | Fwd-AGC GGG ACG CTG TTC TC ; Rev-CCG ACG TGA CAG TAG AGG TAA TAG A | CAG CCC TTC CGC ACC C |
LTBR | Fwd-CAA AGA TGA AGT CAC GGA GGC TAA; Rev-AGA GGA GGT GTT CTG GAA GTG T | CAG CAA CTG TGT CCC C |
TNFα | Fwd-CGT GGA GCT GAA AGA CAA CCA; Rev-AGA GGA CCT GCG AGT AGA TGA G | CAC TGA CGG GCT TTA C |
TNFR1 | Fwd-CTG GTG ATT GTC TTT GGG CTT T; Rev-AGC GAC ATG CTA AAA CCA CAG A | CCT GGC ATC CTT CGC C |
CXCL12 | Fwd-GTC GCC AAA GCC AAC GT; Rev-GGG CAC AGT TTG GAG TGT TGA | AAG CAC CTC AAG ATC C |
CXCR4 | Fwd-CCA TGC CAC CAA CAG TCA GA; Rev-CAG ACA CCA ACA TAG ACC ACC TT | TTC AGC CAG CAG CTT C |
CXCL13 | Fwd-CTG GAG CTC TGC TTC TTA TGC T; Rev-CAG AAC ACC GTG GAC AGG AG | CTG GCC TTC AGC CTC T |
CXCR5 | Fwd-GTC AGT GAA CCC CAT TAC AAC GA; Rev-CAT TGG TTT CGG CTTGGT TCT C | CTC CCT GCC ACA CTG C |
CCL19 | Fwd-CGC CCC ATC CCT GTA TTC C; Rev-CAG CCT GCA GCC ATC CT | CCG CTA CCT GCT CCT C |
CCL21 | Fwd-AGG CCT GGG TGC AGA AC; Rev-CAG TCC TGG GCT GGT TTC C | CCAGACGCCGCATCAG |
CCR7 | Fwd-CTG GAT GCT GGC CAT GGT; Rev-TCT TCT GGA TCC CGC TGT ACA T | CTC CAC CCC AGA GCT G |
LYVE1 | Fwd-ACT TGG ATT AAC TCA TGC TTT CCA GAA; Rev-GTG TAT GTT TCA GTT TCA GCG TTG A | CAC CAC CGA TGA CCC C |
VCAM1 | Fwd-CCA GAT AGA CAG CCC TCT GAA C; Rev- CAG GGT CAA CAT GGA CTT GGA | CTC GCT CCT CAC CTT C |
PrP | Fwd–TGG CAA TGA CTA TGA GGA CCG; Rev- TGG TCT GTA GTA CAC TTG GTT GGG | ACT ATC GTG AAA ACA T |
18S * | Fwd-CGG AAC TGA GGC CAT GAT TAA GAG; Rev- TTC GCT CTG GTC CGT CTT G | TCT AGC GGC GCA ATA C |
Sheep 18S gene was used as housekeeping control gene.