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. 2013 May 3;8(5):e62830. doi: 10.1371/journal.pone.0062830

Table 2. Details of the primers used to determine cytokine, chemokine, and adhesion molecule gene expression levels.

Gene Primer sequences and orientation (5′ to 3′) MGB-FAM probe
LTα Fwd-ATG GCT TCT CTC TCA GCA ACA A ; Rev-AGA CCA CTT GGG AGT AGA CGA A CTC CCT CCT GGT CCC C
LTβ Fwd-AGC GGG ACG CTG TTC TC ; Rev-CCG ACG TGA CAG TAG AGG TAA TAG A CAG CCC TTC CGC ACC C
LTBR Fwd-CAA AGA TGA AGT CAC GGA GGC TAA; Rev-AGA GGA GGT GTT CTG GAA GTG T CAG CAA CTG TGT CCC C
TNFα Fwd-CGT GGA GCT GAA AGA CAA CCA; Rev-AGA GGA CCT GCG AGT AGA TGA G CAC TGA CGG GCT TTA C
TNFR1 Fwd-CTG GTG ATT GTC TTT GGG CTT T; Rev-AGC GAC ATG CTA AAA CCA CAG A CCT GGC ATC CTT CGC C
CXCL12 Fwd-GTC GCC AAA GCC AAC GT; Rev-GGG CAC AGT TTG GAG TGT TGA AAG CAC CTC AAG ATC C
CXCR4 Fwd-CCA TGC CAC CAA CAG TCA GA; Rev-CAG ACA CCA ACA TAG ACC ACC TT TTC AGC CAG CAG CTT C
CXCL13 Fwd-CTG GAG CTC TGC TTC TTA TGC T; Rev-CAG AAC ACC GTG GAC AGG AG CTG GCC TTC AGC CTC T
CXCR5 Fwd-GTC AGT GAA CCC CAT TAC AAC GA; Rev-CAT TGG TTT CGG CTTGGT TCT C CTC CCT GCC ACA CTG C
CCL19 Fwd-CGC CCC ATC CCT GTA TTC C; Rev-CAG CCT GCA GCC ATC CT CCG CTA CCT GCT CCT C
CCL21 Fwd-AGG CCT GGG TGC AGA AC; Rev-CAG TCC TGG GCT GGT TTC C CCAGACGCCGCATCAG
CCR7 Fwd-CTG GAT GCT GGC CAT GGT; Rev-TCT TCT GGA TCC CGC TGT ACA T CTC CAC CCC AGA GCT G
LYVE1 Fwd-ACT TGG ATT AAC TCA TGC TTT CCA GAA; Rev-GTG TAT GTT TCA GTT TCA GCG TTG A CAC CAC CGA TGA CCC C
VCAM1 Fwd-CCA GAT AGA CAG CCC TCT GAA C; Rev- CAG GGT CAA CAT GGA CTT GGA CTC GCT CCT CAC CTT C
PrP Fwd–TGG CAA TGA CTA TGA GGA CCG; Rev- TGG TCT GTA GTA CAC TTG GTT GGG ACT ATC GTG AAA ACA T
18S * Fwd-CGG AAC TGA GGC CAT GAT TAA GAG; Rev- TTC GCT CTG GTC CGT CTT G TCT AGC GGC GCA ATA C
*

Sheep 18S gene was used as housekeeping control gene.