Figure 2. SRPN6 expression following bacterial injection into the mosquito hemocoel.

Approximately 2×10³ bacteria were injected into the hemocoel of adult female An. stephensi. SRPN6 expression was analyzed by Northern blot in the midgut (A) and carcass (all non-gut tissues) (B) 6 h post bacteria injection of into the hemocoel. Procedures and abbreviations are the same as in Figure 1A. Similar results were obtained in three independent experiments. (C) As a control, expression of the anti-microbial peptide defensin was monitored by semi-quantitative RT-PCR in the carcass samples after bacteria injection. Procedures and abbreviations are the same as in Figure 1B.