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. 2013 May 3;8(5):e62895. doi: 10.1371/journal.pone.0062895

Figure 1. The progestin MPA, but not NET-A or P4, induces apoptosis in CD4+ T-cells.

Figure 1

Cells were treated with or without 100 nM Dex, 100 nM F, 1 µM MPA, 10 µM NET-A, 1 µM P4 or vehicle control (EtOH) for 24 hrs. Cells were stained with anti-CD4, anti-CD14, annexin V and 7-AAD using the Apoptosis Detection kit I (BD biosciences). (A) Gating strategy and representative zebra plots of untreated (EtOH), MPA or Dex treated PBMCs. (B) The histogram shows pooled results from two independent experiments with samples from three donors. Data were acquired on a FACS calibur system (BD Biosciences) and analyzed using Flo-Jo software (Tree Star Inc., San Carlos, CA, USA). Statistical significance was determined by one-way ANOVA with Dunnett’s post-test, where *, **, and *** indicate p<0.05, 0.01 and 0.005 respectively. Error bars represent standard deviation.