Figure 4. The GR is involved in Vpr-mediated apoptosis in CD4⁺ T-cells.

PBMCs were treated with 1 µM RU486 in the absence or presence of 5 µM Vpr peptide (amino acids 52–96) for 24 hrs. A tryptic BSA digest served as a control (bars 1 and 2) wherever Vpr peptide was not added and was added at an equivalent mass/volume of peptide. Cells were obtained and stained as described in the methods. The histogram shows pooled results from two independent experiments with samples from three donors. Statistical significance was determined by one-way ANOVA with Dunnett’s post-test or a paired t-test, where *, **, and *** indicate p<0.05, 0.01 and 0.005 respectively. Error bars represent standard deviation.