Table 2. Primers used for real time PCR.

Target Gene	Primer Sequence	Cycling Parameters	Product size (bp)
GAPDH	F: 5′-TGAACGGGAAGCTCACTGG-3′R: 5′-TCCACCACCCTGTTGCTGTA-3′	95°C (10 sec), 55°C (10 sec), 72°C (10 sec)	307
Bcl-2	F: 5′-TTGTGGCCTTCTTTGAGTTCGGTG-3′R: 5′-GTACAGTTCCACAAAGGCATCCCA-3′	95°C (10 sec), 60°C (10 sec), 72°C (10 sec)	167
Bim	F:5′-GAGTGTGACCGAGAAGGTAGACAATTGC-3′R: 5′-CCTTCACCTCCGTGATTGCCTTC-3′	95°C (10 sec), 55°C (10 sec), 72°C (10 sec)	125

F = Forward; R = Reverse.