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. 2013 May 3;8(5):e62768. doi: 10.1371/journal.pone.0062768

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© 2013 Schulz et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Extracted ion chromatograms corresponding to the un- (full) mono- (dashed) and di- (dotted) glycosylated versions of the Leu358-Lys387 tryptic peptide containing the AniA glycosylation sites: (A) wild type, (B) S373A, (C) S378A, (D) S373A, S378A, (E) S373A, S378A, S385P. Corresponding variant sequences are shown in Table S6. (F) Proportion of un- (white) mono- (gray) or di- (black) glycosylated versions of each variant shown in (A)–(E), as determined by integration of extracted ion chromatograms. Values are mean, error bars show s.e.m. *, P = 0.01 2-sided Mann-Whitney test.