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. 2013 May 3;8(5):e62524. doi: 10.1371/journal.pone.0062524

Figure 1. Activation of cAMP pathway modulated E2-stimulated lhcgr expression in a biphasic manner.

Figure 1

The cells were co-treated with forskolin (10 µM) or db-cAMP (1 mM) and E2 (50 nM) for 3 h (A and C) or 24 h (B and D) before the end of the 24-h treatment period. The follicle cells in each well were lyzed directly in TRI-Reagent for RNA extraction, RT and real-time qPCR to analyze the mRNA levels lhcgr and the house-keeping gene ef1a. The data were expressed as fold change compared to the control group after normalization to the expression of ef1a. Different letters in each data set indicated statistical significance (P<0.05; mean ± SEM, n = 3–4).