Figure 4. Pre-activation of cAMP enhanced E2-stimulated lhcgr expression likely by promoting follicle cell responsiveness to E2.

Cells were administered with forskolin (10 µM) or db-cAMP (1 mM) for 24 h before a 3-h treatment of E2 (50 nM). Relative mRNA levels of (A) lhcgr, (B) esr1, esr2a and esr2b were expressed as fold change compared to the control group after normalization to the expression of ef1a. Different letters in each data set indicated statistical significance (P<0.05; mean ± SEM, n = 3).