Figure 5. Both basal and E2-induced lhcgr expression were highly dependent on PKC pathway.

Effect of GF109203X (A and B) and Ro-31-8220 (C and D) on basal and E2-stimulated lhcgr expression at 3 h and 24 h of treatment in cultured zebrafish follicle cells. The cells were pretreated with GF109203X (10 µM) or Ro-31-8220 (1 µM) for 15 min followed by treatment with E2 (50 nM) for 3 h or 24 h before the end of the 24-h treatment period. The data were expressed as fold change compared to the control group after normalization to the expression of ef1a. Different letters in each data set indicated statistical significance (P<0.05; mean ± SEM, n = 3–4).