Figure 6. E2 could not directly activate PKC in zebrafish cultured follicle cells.

The cells were treated with PKC activator, PMA (100 nM) or E2 (50 nM) for 20 min before the end of the 24-h treatment period. The treated cells were fractionated into cytosol (Cyto) and membrane (Mem) protein fractions followed by SDS-PAGE and Western blot analysis against phospho-PKCα/βII (p-PKCα/βII), p44/42 MAPK (cytosol marker) and pan-cadherin (membrane marker).