Figure 7. PKC pathway was crucial for nuclear estrogen receptor expression.

The cultured follicle cells were pretreated with GF109203X (10 µM) or Ro-31-8220 (1 µM) for 15 min followed by treatment with E2 (50 nM) for 3 h (A and C) or 24 h (B and D) before the end of the 24-h treatment period. Quantification of mRNA level of esr1, esr2a and esr2b was carried out. The data were expressed as fold change compared to the control group after normalization to the expression of ef1a. Different letters in each data set indicated statistical significance (P<0.05; mean ± SEM, n = 3–4).