Table 2. Primer and MgCl2 concentrations in qPCR reactions.
| Gene symbol | Gene name | Forward primer (5′->3′) | Reverse primer (5′->3′) | [Primer] (µM) | [MgCl2] (mM) |
| KRT19 | Keratin 19 | GATGAGCAGGTCCGAGGTTA | TCTTCCAAGGCAGCTTTCAT | 0.3 | 2.00 |
| CEACAM5 | Carcinoembryonic antigen-related cell adhesion molecule 5 | GGGACCTATGCCTGTTTTGTCTC | GAGCAACCCCAACCAGCAC | 0.2 | 1.25 |
| EPCAM | Epithelial cell adhesion molecule | CGCAGCTCAGGAAGAATGTG | TGAAGTACACTGGCATTGACG | 0.3 | 1.25 |
| DSG3 | Desmoglein 3 | GGCAAAAACGTGAATGGGTGA | GGGTTGCTTGGTAATCTGAAGTA | 0.3 | 1.75 |
| SFTPA | Surfactant protein A | TTGGAGGCAGAGACCCAAGCAG | GGCTCCAAGAAATCAGCGACCC | 0.3 | 1.25 |
| SFTPB | Surfactant protein B | GTCCAGCCCTCTCCAGTGTATC | GCCCGTCTCACTTGGCTTTTC | 0.3 | 2.00 |
| SFTPC | Surfactant protein C | AGCAAAGAGGTCCTGATGGA | ACAATCACCACGACGATGAG | 0.3 | 1.25 |
| BCR | Breakpoint cluster region | GCTCTATGGGTTTCTGAATG | AAATACCCAAAGGAATCCAC | 0.15 | 2.00 |