Figure 3. Berberine prevents morphological disruption of tight junction induced by IFN-γ and TNF-α.

Caco-2 monolayers were treated as described in Fig. 1A. Tight junction proteins ZO-1, occludin and claudin-1 were stained by immunofluorescence. Berberine dramatically prevented the IFN-γ and TNF-α-induced morphological disruption of tight junction proteins ZO-1, occludin and claudin-1 in Caco-2 monolayers. Data are representative of four independent experiments. Scale bar = 10 µm.