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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Cancer Res. 2013 Jan 29;73(9):2806–2816. doi: 10.1158/0008-5472.CAN-12-2374

Figure 1. Treatment of HT-29 cells with cPAF decreases proliferation of colon cancer cells.

Figure 1

A. HT-29 cells seeded in 96-well plates were allowed to grow for 48 hours in media containing 10% FBS. The growth medium then was removed and the cells were exposed to the indicated concentrations of cPAF in media containing 0.1% FBS. After 72 hours at 37°C, we assessed cellular proliferation using the XTT assay, as described in Materials and Methods. *, p<0.05; **, p<0.005.

B. HT-29 cells seeded in 96-well plates were allowed to grow for 24 hours in media containing 10% FBS. The growth medium then was removed and the cells were exposed to the indicated concentrations of cPAF and BN-52021 in serum-free medium. Fresh media containing cPAF and BN-52021 were supplied every 24 hours. After a total of 72 hours at 37°C, we assessed cellular proliferation using the XTT assay. *, p<0.05.