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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Cancer Res. 2013 Jan 29;73(9):2806–2816. doi: 10.1158/0008-5472.CAN-12-2374

Figure 3. cPAF treatment decreases basal pAkt473 levels.

Figure 3

A. Starved HT-29 cells were treated with 1.5 μM cPAF or vehicle for 10, 30, and 240 min at 37°C. The levels of pAkt473, Akt, and β-actin then were determined in protein lysates, using immunoblot analyses.

B. Starved HCT-116 and LoVo cells were treated with 1.5 μM cPAF or vehicle for 30 min at 37°C. The levels of pAkt473, Akt, and β-actin then were determined using immunoblot analyses, as above.

C. Starved HT-29 cells were treated with cPAF or vehicle for 30 min at 37°C. The levels of pAkt308, pAkt473, Akt and β-actin then were determined as above.