Figure 1.
Crosslinking [ubiquitin +7H]7+ with [sulfo-EGS-H]−. (a) nESI mass spectrum ubiquitin. (b) Product ion spectrum of the ion/ion reaction between [ubiquitin +7H]7+ and [sulfo-EGS-H]−. (c) CID product ion spectrum of the [M+6H+sulfo-EGS]6+ ion. (d) CID product ion spectrum of the [M+6H+sulfo-EGS - sulfo-NHS]6+ ion (i.e. ions indicated as –sulfo-NHS) from (c).