Figure 4.

Short-term 1400W treatment during tumor initiation reduces DEN-induced tumorigenesis in GSNOR^−/− mice. DEN-induced tumorigenesis as described in Fig. 3A was analyzed in the following four groups: wild-type (WT) and GSNOR^−/− (KO) mice treated with 1400W or PBS. Each data point represents an individual animal, and the mean of each group is represented by a line. A, Number of tumors (≥ 2mm) on the liver surface in each animal. The tumor number was significantly higher in the PBS-treated KO mice compared to each of the other three groups (P < 8 × 10⁻⁵), which was not significantly different from each other. B, The maximal tumor diameter was significantly higher in the PBS-treated KO mice compared to each of the other three groups (P < 0.009). C, The tumor burden, calculated as the sum of the areas of surface tumors, was significantly higher in the PBS-treated KO mice compared to each of the other three groups (P < 0.003).