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. 2013 Apr 9;4:1682. doi: 10.1038/ncomms2673

Figure 8. Piezo2 is required for allodynia in two models of chronic neuropathic pain.

Figure 8

Mice were subjected to a unilateral L5 spinal nerve transaction (L5 SNT; a) or to a unilateral chronic constriction injury of the sciatic nerve (CCI; b). The 50% threshold to von Frey was measured at the ipsilateral and contralateral paw. Piezo2 (L5 SNT, n=5; CCI, n=6) or mismatch antisense (L5 SNT, n=9; CCI, n=10) was administered after full development of allodynia (2–3 weeks after operation) as indicated by the grey bars. (c) Fold reduction of 50% threshold to von Frey of the ipsilateral paw compared the contralateral paw in both models of neuropathic pain at day 21 (L5 SNT; n=5–9) or 26 (CCI; n=6–10). Piezo2 mRNA expression in the ipsi- and contralateral DRG expression was measured 1 day after the last antisense ODN administration(d, L 5 SNT, n=5–9; e, CCI, n=6–10). Two-way one-way analysis of variance showed a significant overall reduction in Piezo2 mRNA P<0.01. Data are expressed as mean±s.e.m. Data were analysed using two-way analysis of variance followed by the Bonferroni post hoc test. *P<0.05, **P<0.001, ***P<0.001. In a and b ‘###’indicate P<0.001 compared to the contralateral paw of mismatch antisense ODN treated mice.