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. 2013 Apr 23;4:1769. doi: 10.1038/ncomms2742

Figure 6. miR-155 regulates cold-induced thermogenesis in BAT and ‘browning’ of WAT.

Figure 6

(a) Representative infrared thermographic image of 12–16-week-old male wt and miR-155−/− littermates kept at 4 °C for 4 h. (b) Statistical analysis of body surface temperature as measured by infrared thermography. Data are represented as means±s.e.m. (***P<0.001; Student’s t-test; n.s., not significant; wt group n=7, miR-155−/− group n=5). (c) Hematoxylin and eosin staining of interscapular BAT sections from wt and miR-155−/− littermates kept at room tempertaure (RT) or post cold exposure (4 °C, 4 h). Scale bar, 50 μm. (d) Lipolysis assay of BAT from 12-week-old wt and miR-155−/− littermates kept in 4 °C for 4 h. Data are represented as means±s.e.m. (*P<0.05; Student’s t-test; n=4). (e) Ex vivo oxygraph measurement of cellular mitochondrial respiration in BAT 12–16-weeks-old wt or miR-155−/− littermates exposed to 4 °C. Data are represented as means±s.e.m. (*P<0.05; Student’s t-test; wt group n=6, miR-155−/− group n=5). (f) qRT–PCR analyses of UCP1 and PGC-1α expression in BAT of 12–16-week-old wt or miR-155−/− littermates after cold exposure. Data are represented as means±s.e.m. (*P<0.05; Student’s t-test; n=4). (g) Representative hematoxylin and eosin staining of igWAT sections from wt and miR-155−/− littermates at RT or post cold exposure (top). Immunohistochemical staining for UCP1 abundance in respective igWAT sections (bottom). Scale bar, 50 μm. (h) qRT–PCR analyses of UCP1 and PGC-1α in igWAT 12–16-week-old wt or miR-155−/− littermates exposed to 4 °C for 4 h. Data are represented as means±s.e.m. (*P<0.05; Student’s t-test; wt group n=4, miR-155−/− group n=4). (i) Oxygraph measurement of cellular mitochondrial respiration in igWAT of wt and miR-155−/− littermates at 12–16 weeks of age. Data are represented as means±s.e.m. (*P<0.05; Student’s t-test; wt group n=6, miR-155−/− group n=5).