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. 1992 May;12(5):2383–2390. doi: 10.1128/mcb.12.5.2383

B-cell factor 1 is required for optimal expression of the DRA promoter in B cells.

C F Voliva 1, A Aronheim 1, M D Walker 1, B M Peterlin 1
PMCID: PMC364410  PMID: 1569956

Abstract

The X box in the DRA promoter of the human histocompatibility complex is required for expression of the DRA gene in B cells. We show that a B-cell factor binds to a sequence that is clearly distinguishable from binding sites for the previously described X box binding nuclear proteins RF-X, NF-X, NF-Xc, NF-S, hXBP, and AP-1. Mutations in the DRA X box that disrupt the binding of this factor result in a lower level of gene expression, as does the presence of Id (a trans-dominant regulatory protein that negatively regulates helix-loop-helix proteins). Furthermore, this factor is recognized by antibodies directed against the helix-loop-helix protein A1, a mouse homolog of the immunoglobulin enhancer binding proteins E12/E47, and it binds to sequences in other genes that were previously shown to bind these proteins. By these criteria, this factor is BCF-1.

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Selected References

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