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. Author manuscript; available in PMC: 2013 May 4.
Published in final edited form as: J Immunol. 2009 Dec 14;184(2):956–964. doi: 10.4049/jimmunol.0902008

Figure 6. TcpB promotes enhanced polyubiquitination of MAL.

Figure 6

293 cells were transfected with HA-MAL, VSV-Ubiquitin and TcpB using PEI. After 24 hrs, cells were treated with 20 mM MG132 where indicated for additional 4 hrs. Cell lysates were then immunoprecipitated with a-HA as described in methods and materials and immunoblotted with anti-VSV for detection of polyubiquitinated MAL.