Fig. 2.

Isolation and characterization of mouse retinal EC. (A) The Eng+/+ and Eng+/− EC were isolated and cultured on gelatin-coated plates. Cells were photographed in digital format under subconfluence and confluence conditions. (B) Expression of vascular endothelial cell markers. Retinal EC were examined for expression of PECAM-1, endoglin, B4-lectin, VCAM-1, ICAM-1, ICAM-2, VEGFR1, and VEGFR2 by FACS analysis. The shaded areas show control IgG staining. These experiments were repeated twice with two different isolations of EC with similar results. Representative geometrical means of the expression are noted.