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. 2013 Mar 15;126(6):1406–1415. doi: 10.1242/jcs.117614

Fig. 3.

Fig. 3.

dPPP1R15 is not regulated transcriptionally by ER stress. (A) Transcriptional fold-changes of dPPP1R15, HSC3 and ERO1L mRNA measured by qPCR after 6 hours of treatment with 2 mM DTT and expressed relative to RP49. Means + s.e.m. are shown; n = 3 independent repeats, *P<0.05, **P<0.01 compared with untreated control. (B) Representative fluorescence images of salivary glands of early 3rd instar larvae. Salivary gland specific expression of either XBP1-eGFP reporter alone or coexpressed with Fkh-Gal4>UAS-dPPP1R15 Fkh-Gal4>UAS-dPERK RNAi (v16427). Salivary glands were dissected from early 3rd instar larvae and tissue further incubated at room temperature in Express Five® SFM media with or without addition of DTT (final 2 mM) for 3 hours. After tissue fixation, and mounting, the appearance of GFP signal was analysed using a LSM510 META (Zeiss) fluorescence confocal microscope. Flies were reared at 25°C. (C) Quantification of data from B.