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. 2013 Mar 15;126(6):1464–1476. doi: 10.1242/jcs.120915

Fig. 1.

Fig. 1.

Tspan18 and Cad6B expression in cranial premigratory neural crest cells is downregulated prior to migration. (A–H) Whole mount in situ hybridization for Tspan18 (A–D) or Cad6B (E–H) in embryos at 5 somite (s; A,E), 6 somites (B,F), 7 somites (C,G) and 8 somites (D,H) (dorsal view, anterior embryo half). Tspan18 and Cad6B expression in the cranial dorsal neural tube (A–C,E,F; arrowheads) is downregulated (D,G,H; arrows) prior to neural crest migration. Tspan18 and Cad6B expression in the forebrain (brackets in D,H) persists during midbrain neural crest migration. Tspan18 and Cad6B are also expressed in the developing vasculature (asterisks in B,F) and Tspan18 is expressed in the head mesenchyme and epithelial somites. (A′–H′). Transverse sections at the levels indicated in A–H reveal Tspan18 (A′–D′) and Cad6B (E′–H′) expression in premigratory neural crest cells in the dorsal neural tube (arrowheads in A′–C′ and E′,F′) that is downregulated upon neural crest migration (D′,G′,H′; arrows). Tspan18 expression in the cranial mesenchyme is also apparent. fb, forebrain; m, mesenchyme; nt, neutral tube; som, somite.