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. 2011 Nov;90(5):855–865. doi: 10.1189/jlb.0211062

Figure 6. i.t. administration of rTRAIL induces neutrophil apoptosis and reduces neutrophil numbers in vivo.

Figure 6.

TRAIL (5 μg) was administered i.t. 24 h after nebulized administration of 3 mg LPS; n = 6 animals in each group. Total neutrophil counts at 48 h from WT mice are shown as open bars and from TRAIL-deficient mice as filled bars. There were significantly more (A) cells and (B) neutrophils in the TRAIL-deficient mice lavages when compared with WT, which significantly reduced in response to TRAIL therapy. (C) At 48 h, the percentage of apoptotic neutrophils was reduced in TRAIL-deficient mice compared with WT mice but significantly increased in response to TRAIL treatment. Histologic sections of WT mice 24 h post-TRAIL treatment were TUNEL-stained for detection of apoptotic events. (D) Numbers of TUNEL-positive cells were significantly higher in WT (E and F) mice following TRAIL treatment (tx). Neb, Nebulized.