Figure 4.

The ngTMA process: strategic and operative levels. The first step in the construction of ngTMAs is the design and planning in terms of the scientific hypothesis, available material, case selection, as well as TMA and statistical considerations. The operative level begins by slide scanning and the use of a digital platform. Histological expertise is necessary to perform precise annotations. Automated tissue microarraying ensues. The final product is an optimized ngTMA which will allow the user to answer the hypothesis in question.