Figure 1.

Screenshots of SDM-Assist while creating primers to mutate the Arabidopsis potassium channel AKT1. (A) Dragging and dropping of Alanine onto the Glycine residues at position 255 and 257 (black arrow) of the AKT1 sequence creates a stretch of 9 amino acids (below, “Mutated site”) that includes Alanine (orange) instead of Glycine (yellow) residues. Pressing the “Mutagenise” button opens a new window (B) that includes all possible nucleotide sequences derived from those 9 amino acids. The table highlights the mutated nucleotides in red and resulting palindromes which create a restriction site in green. The user has to choose one of the mutated sequences and press the button “primerise” to get a list of potential primers (see Table 1).